Construct: shRNA TRCN0000432583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006731.2-735s21c1
DNA Barcode:
AGTTACAGTTTGGTCGTTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FKTN (2218)
Gene Description:
fukutin
Transcript:
RefSeq NM_006731.2 (CURRENT)
Match location:
Position 735 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTACAGTTTGGTCGTTATC
Hairpin Sequence:
5'-CCGG-AGTTACAGTTTGGTCGTTATC-CTCGAG-GATAACGACCAAACTGTAACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTACAGTTTGGTCGTTATCCTCGAGGATAACGACCAAACTGTAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTACAGTTTGGTCGTTATCCTCGAGGATAACGACCAAACTGTAACT-3'

Other clones with same target sequence:

(none)