Construct: shRNA TRCN0000432598

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004980.3-1936s21c1
DNA Barcode:
ACCACTGGGTTGTCCTATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCND3 (3752)
Gene Description:
potassium voltage-gated channel subfamily D member 3
Transcript:
RefSeq NM_004980.3 (NON-CURRENT)
Match location:
Position 1936 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432598)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCACTGGGTTGTCCTATCTT
Hairpin Sequence:
5'-CCGG-ACCACTGGGTTGTCCTATCTT-CTCGAG-AAGATAGGACAACCCAGTGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCACTGGGTTGTCCTATCTTCTCGAGAAGATAGGACAACCCAGTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCACTGGGTTGTCCTATCTTCTCGAGAAGATAGGACAACCCAGTGGT-3'

Other clones with same target sequence:

(none)