Construct: shRNA TRCN0000432819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183374.2-748s21c1
DNA Barcode:
GCCATTTCTGAGAAGCTTCAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP26C1 (340665)
Gene Description:
cytochrome P450 family 26 subfamily C member 1
Transcript:
RefSeq NM_183374.2 (NON-CURRENT)
Match location:
Position 748 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTTCTGAGAAGCTTCAC
Hairpin Sequence:
5'-CCGG-GCCATTTCTGAGAAGCTTCAC-CTCGAG-GTGAAGCTTCTCAGAAATGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTTCTGAGAAGCTTCACCTCGAGGTGAAGCTTCTCAGAAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTTCTGAGAAGCTTCACCTCGAGGTGAAGCTTCTCAGAAATGGC-3'

Other clones with same target sequence:

(none)