Construct: shRNA TRCN0000432972

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153456.2-1587s21c1
DNA Barcode:
TTTATCCAGCTGGAGATTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HS6ST3 (266722)
Gene Description:
heparan sulfate 6-O-sulfotransferase 3
Transcript:
RefSeq NM_153456.2 (NON-CURRENT)
Match location:
Position 1587 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432972)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTATCCAGCTGGAGATTATC
Hairpin Sequence:
5'-CCGG-TTTATCCAGCTGGAGATTATC-CTCGAG-GATAATCTCCAGCTGGATAAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTATCCAGCTGGAGATTATCCTCGAGGATAATCTCCAGCTGGATAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTATCCAGCTGGAGATTATCCTCGAGGATAATCTCCAGCTGGATAAA-3'

Other clones with same target sequence:

(none)