Construct: shRNA TRCN0000433006

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004977.2-2922s21c1
DNA Barcode:
GCCTTCTTGGACCCTTTAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNC3 (3748)
Gene Description:
potassium voltage-gated channel subfamily C member 3
Transcript:
RefSeq NM_004977.2 (NON-CURRENT)
Match location:
Position 2922 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433006)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCTTGGACCCTTTAAAG
Hairpin Sequence:
5'-CCGG-GCCTTCTTGGACCCTTTAAAG-CTCGAG-CTTTAAAGGGTCCAAGAAGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCTTGGACCCTTTAAAGCTCGAGCTTTAAAGGGTCCAAGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCTTGGACCCTTTAAAGCTCGAGCTTTAAAGGGTCCAAGAAGGC-3'

Other clones with same target sequence:

(none)