Construct: shRNA TRCN0000433033

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032718.3-1933s21c1
DNA Barcode:
ACTTGAGTTTCCAGTTAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MFSD9 (84804)
Gene Description:
major facilitator superfamily domain containing 9
Transcript:
RefSeq NM_032718.3 (NON-CURRENT)
Match location:
Position 1933 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433033)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTGAGTTTCCAGTTAAATT
Hairpin Sequence:
5'-CCGG-ACTTGAGTTTCCAGTTAAATT-CTCGAG-AATTTAACTGGAAACTCAAGT-TTTTTTTGAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTGAGTTTCCAGTTAAATTCTCGAGAATTTAACTGGAAACTCAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTGAGTTTCCAGTTAAATTCTCGAGAATTTAACTGGAAACTCAAGT-3'

Other clones with same target sequence:

(none)