Construct: shRNA TRCN0000433132

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020340.4-3942s21c1
DNA Barcode:
CCGACCTTTCGAGCGCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARFGEF3 (57221)
Gene Description:
ARFGEF family member 3
Transcript:
RefSeq NM_020340.4 (NON-CURRENT)
Match location:
Position 3942 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433132)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACCTTTCGAGCGCATTAT
Hairpin Sequence:
5'-CCGG-CCGACCTTTCGAGCGCATTAT-CTCGAG-ATAATGCGCTCGAAAGGTCGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACCTTTCGAGCGCATTATCTCGAGATAATGCGCTCGAAAGGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACCTTTCGAGCGCATTATCTCGAGATAATGCGCTCGAAAGGTCGG-3'

Other clones with same target sequence:

(none)