Construct: shRNA TRCN0000433146

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182703.3-129s21c1
DNA Barcode:
CTGATTGGGAGGAGGGTTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKDD1A (348094)
Gene Description:
ankyrin repeat and death domain containing 1A
Transcript:
RefSeq NM_182703.3 (NON-CURRENT)
Match location:
Position 129 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433146)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGATTGGGAGGAGGGTTAAC
Hairpin Sequence:
5'-CCGG-CTGATTGGGAGGAGGGTTAAC-CTCGAG-GTTAACCCTCCTCCCAATCAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGATTGGGAGGAGGGTTAACCTCGAGGTTAACCCTCCTCCCAATCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGATTGGGAGGAGGGTTAACCTCGAGGTTAACCCTCCTCCCAATCAG-3'

Other clones with same target sequence:

(none)