Construct: shRNA TRCN0000433239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019015.1-3324s21c1
DNA Barcode:
TCAACCGCTGTCGCATGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHPF2 (54480)
Gene Description:
chondroitin polymerizing factor 2
Transcript:
RefSeq NM_019015.1 (NON-CURRENT)
Match location:
Position 3324 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAACCGCTGTCGCATGAATG
Hairpin Sequence:
5'-CCGG-TCAACCGCTGTCGCATGAATG-CTCGAG-CATTCATGCGACAGCGGTTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAACCGCTGTCGCATGAATGCTCGAGCATTCATGCGACAGCGGTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAACCGCTGTCGCATGAATGCTCGAGCATTCATGCGACAGCGGTTGA-3'

Other clones with same target sequence:

(none)