Construct: shRNA TRCN0000433347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138778.2-1433s21c1
DNA Barcode:
AGAAGCAGACTCAGCCTTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPH7 (92715)
Gene Description:
diphthamide biosynthesis 7
Transcript:
RefSeq NM_138778.2 (NON-CURRENT)
Match location:
Position 1433 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGCAGACTCAGCCTTCAG
Hairpin Sequence:
5'-CCGG-AGAAGCAGACTCAGCCTTCAG-CTCGAG-CTGAAGGCTGAGTCTGCTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGCAGACTCAGCCTTCAGCTCGAGCTGAAGGCTGAGTCTGCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGCAGACTCAGCCTTCAGCTCGAGCTGAAGGCTGAGTCTGCTTCT-3'

Other clones with same target sequence:

(none)