Construct: shRNA TRCN0000433360

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002977.3-5164s21c1
DNA Barcode:
GAATCCTACGTCTAGTCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCN9A (6335)
Gene Description:
sodium voltage-gated channel alpha subunit 9
Transcript:
RefSeq NM_002977.3 (CURRENT)
Match location:
Position 5164 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433360)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCCTACGTCTAGTCAAAG
Hairpin Sequence:
5'-CCGG-GAATCCTACGTCTAGTCAAAG-CTCGAG-CTTTGACTAGACGTAGGATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCCTACGTCTAGTCAAAGCTCGAGCTTTGACTAGACGTAGGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCCTACGTCTAGTCAAAGCTCGAGCTTTGACTAGACGTAGGATTC-3'

Other clones with same target sequence:

(none)