Construct: shRNA TRCN0000433459

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004171.3-2510s21c1
DNA Barcode:
TGCTTTATAACCGTCTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC1A2 (6506)
Gene Description:
solute carrier family 1 member 2
Transcript:
RefSeq NM_004171.3 (NON-CURRENT)
Match location:
Position 2510 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433459)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTTTATAACCGTCTAATTT
Hairpin Sequence:
5'-CCGG-TGCTTTATAACCGTCTAATTT-CTCGAG-AAATTAGACGGTTATAAAGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTTTATAACCGTCTAATTTCTCGAGAAATTAGACGGTTATAAAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTTTATAACCGTCTAATTTCTCGAGAAATTAGACGGTTATAAAGCA-3'

Other clones with same target sequence:

(none)