Construct: shRNA TRCN0000433490
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_017923.3-1134s21c1
- DNA Barcode:
- GCGTAAAGTAAAGTATGATAT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MARCHF1 (55016)
- Gene Description:
- membrane associated ring-CH-type finger 1
- Transcript:
- RefSeq NM_017923.3 (NON-CURRENT)
- Match location:
- Position 1134 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000433490)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- GCGTAAAGTAAAGTATGATAT
- Hairpin Sequence:
- 5'-CCGG-GCGTAAAGTAAAGTATGATAT-CTCGAG-ATATCATACTTTACTTTACGC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGTAAAGTAAAGTATGATATCTCGAGATATCATACTTTACTTTACGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGTAAAGTAAAGTATGATATCTCGAGATATCATACTTTACTTTACGC-3'