Construct: shRNA TRCN0000433499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008646.3-1401s21c1
DNA Barcode:
GCTACATCTACCTTGTCAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mug2 (17837)
Gene Description:
murinoglobulin 2
Transcript:
RefSeq NM_008646.3 (CURRENT)
Match location:
Position 1401 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACATCTACCTTGTCAAAG
Hairpin Sequence:
5'-CCGG-GCTACATCTACCTTGTCAAAG-CTCGAG-CTTTGACAAGGTAGATGTAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACATCTACCTTGTCAAAGCTCGAGCTTTGACAAGGTAGATGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACATCTACCTTGTCAAAGCTCGAGCTTTGACAAGGTAGATGTAGC-3'

Other clones with same target sequence:

(none)