Construct: shRNA TRCN0000433610

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018945.3-2120s21c1
DNA Barcode:
TGAGAGAAAGTACCTTCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDE7B (27115)
Gene Description:
phosphodiesterase 7B
Transcript:
RefSeq NM_018945.3 (NON-CURRENT)
Match location:
Position 2120 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433610)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGAGAAAGTACCTTCTATT
Hairpin Sequence:
5'-CCGG-TGAGAGAAAGTACCTTCTATT-CTCGAG-AATAGAAGGTACTTTCTCTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGAGAAAGTACCTTCTATTCTCGAGAATAGAAGGTACTTTCTCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGAGAAAGTACCTTCTATTCTCGAGAATAGAAGGTACTTTCTCTCA-3'

Other clones with same target sequence:

(none)