Construct: shRNA TRCN0000433631

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173479.3-699s21c1
DNA Barcode:
GGATCATGGAATCTGCATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR88 (126248)
Gene Description:
WD repeat domain 88
Transcript:
RefSeq NM_173479.3 (NON-CURRENT)
Match location:
Position 699 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433631)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATCATGGAATCTGCATAAT
Hairpin Sequence:
5'-CCGG-GGATCATGGAATCTGCATAAT-CTCGAG-ATTATGCAGATTCCATGATCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATCATGGAATCTGCATAATCTCGAGATTATGCAGATTCCATGATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATCATGGAATCTGCATAATCTCGAGATTATGCAGATTCCATGATCC-3'

Other clones with same target sequence:

(none)