Construct: shRNA TRCN0000433641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017575.4-2403s21c1
DNA Barcode:
TTGACGCTGTCTATTACTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMG6 (23293)
Gene Description:
SMG6 nonsense mediated mRNA decay factor
Transcript:
RefSeq NM_017575.4 (NON-CURRENT)
Match location:
Position 2403 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGACGCTGTCTATTACTATA
Hairpin Sequence:
5'-CCGG-TTGACGCTGTCTATTACTATA-CTCGAG-TATAGTAATAGACAGCGTCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGACGCTGTCTATTACTATACTCGAGTATAGTAATAGACAGCGTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGACGCTGTCTATTACTATACTCGAGTATAGTAATAGACAGCGTCAA-3'

Other clones with same target sequence:

(none)