Construct: shRNA TRCN0000433699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_079420.2-327s21c1
DNA Barcode:
TGATTCCAAGATCACCTTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYL1 (4632)
Gene Description:
myosin light chain 1
Transcript:
RefSeq NM_079420.2 (NON-CURRENT)
Match location:
Position 327 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATTCCAAGATCACCTTAAG
Hairpin Sequence:
5'-CCGG-TGATTCCAAGATCACCTTAAG-CTCGAG-CTTAAGGTGATCTTGGAATCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATTCCAAGATCACCTTAAGCTCGAGCTTAAGGTGATCTTGGAATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATTCCAAGATCACCTTAAGCTCGAGCTTAAGGTGATCTTGGAATCA-3'

Other clones with same target sequence:

(none)