Construct: shRNA TRCN0000433813

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033034.2-434s21c1
DNA Barcode:
AGAAGTCCATGCTAGACAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM5 (85363)
Gene Description:
tripartite motif containing 5
Transcript:
RefSeq NM_033034.2 (NON-CURRENT)
Match location:
Position 434 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433813)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGTCCATGCTAGACAAAG
Hairpin Sequence:
5'-CCGG-AGAAGTCCATGCTAGACAAAG-CTCGAG-CTTTGTCTAGCATGGACTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGTCCATGCTAGACAAAGCTCGAGCTTTGTCTAGCATGGACTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGTCCATGCTAGACAAAGCTCGAGCTTTGTCTAGCATGGACTTCT-3'

Other clones with same target sequence:

(none)