Construct: shRNA TRCN0000433819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014901.4-1516s21c1
DNA Barcode:
GATGTGGAGATGGAGAACTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF44 (22838)
Gene Description:
ring finger protein 44
Transcript:
RefSeq NM_014901.4 (NON-CURRENT)
Match location:
Position 1516 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTGGAGATGGAGAACTAT
Hairpin Sequence:
5'-CCGG-GATGTGGAGATGGAGAACTAT-CTCGAG-ATAGTTCTCCATCTCCACATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTGGAGATGGAGAACTATCTCGAGATAGTTCTCCATCTCCACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTGGAGATGGAGAACTATCTCGAGATAGTTCTCCATCTCCACATC-3'

Other clones with same target sequence:

(none)