Construct: shRNA TRCN0000433833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018838.3-376s21c1
DNA Barcode:
ATTCATTTGGACGAACCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDUFA12 (55967)
Gene Description:
NADH:ubiquinone oxidoreductase subunit A12
Transcript:
RefSeq NM_018838.3 (NON-CURRENT)
Match location:
Position 376 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCATTTGGACGAACCATAA
Hairpin Sequence:
5'-CCGG-ATTCATTTGGACGAACCATAA-CTCGAG-TTATGGTTCGTCCAAATGAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCATTTGGACGAACCATAACTCGAGTTATGGTTCGTCCAAATGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCATTTGGACGAACCATAACTCGAGTTATGGTTCGTCCAAATGAAT-3'

Other clones with same target sequence:

(none)