Construct: shRNA TRCN0000433871

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018353.4-4016s21c1
DNA Barcode:
TCATTGCTGCAGCTTACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MIS18BP1 (55320)
Gene Description:
MIS18 binding protein 1
Transcript:
RefSeq NM_018353.4 (NON-CURRENT)
Match location:
Position 4016 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433871)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATTGCTGCAGCTTACTAAA
Hairpin Sequence:
5'-CCGG-TCATTGCTGCAGCTTACTAAA-CTCGAG-TTTAGTAAGCTGCAGCAATGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATTGCTGCAGCTTACTAAACTCGAGTTTAGTAAGCTGCAGCAATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATTGCTGCAGCTTACTAAACTCGAGTTTAGTAAGCTGCAGCAATGA-3'

Other clones with same target sequence:

(none)