Construct: shRNA TRCN0000433901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020809.2-1918s21c1
DNA Barcode:
GAGAATTGCCTTAGGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP20 (57569)
Gene Description:
Rho GTPase activating protein 20
Transcript:
RefSeq NM_020809.2 (NON-CURRENT)
Match location:
Position 1918 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAATTGCCTTAGGATATTT
Hairpin Sequence:
5'-CCGG-GAGAATTGCCTTAGGATATTT-CTCGAG-AAATATCCTAAGGCAATTCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAATTGCCTTAGGATATTTCTCGAGAAATATCCTAAGGCAATTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAATTGCCTTAGGATATTTCTCGAGAAATATCCTAAGGCAATTCTC-3'

Other clones with same target sequence:

(none)