Construct: shRNA TRCN0000433903

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000668.4-1592s21c1
DNA Barcode:
CATGCTGGGCCATTGTGATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADH1B (125)
Gene Description:
alcohol dehydrogenase 1B (class I), beta polypeptide
Transcript:
RefSeq NM_000668.4 (NON-CURRENT)
Match location:
Position 1592 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433903)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGCTGGGCCATTGTGATTG
Hairpin Sequence:
5'-CCGG-CATGCTGGGCCATTGTGATTG-CTCGAG-CAATCACAATGGCCCAGCATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGCTGGGCCATTGTGATTGCTCGAGCAATCACAATGGCCCAGCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGCTGGGCCATTGTGATTGCTCGAGCAATCACAATGGCCCAGCATG-3'

Other clones with same target sequence:

(none)