Construct: shRNA TRCN0000433910

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003323.2-708s21c1
DNA Barcode:
ATGCACACAAGTCACCCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TULP2 (7288)
Gene Description:
TUB like protein 2
Transcript:
RefSeq NM_003323.2 (NON-CURRENT)
Match location:
Position 708 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433910)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCACACAAGTCACCCAATA
Hairpin Sequence:
5'-CCGG-ATGCACACAAGTCACCCAATA-CTCGAG-TATTGGGTGACTTGTGTGCAT-TTTTTTTGAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCACACAAGTCACCCAATACTCGAGTATTGGGTGACTTGTGTGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCACACAAGTCACCCAATACTCGAGTATTGGGTGACTTGTGTGCAT-3'

Other clones with same target sequence:

(none)