Construct: shRNA TRCN0000433994

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001999.3-1995s21c1
DNA Barcode:
GTGAATGCAACATGGGTTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBN2 (2201)
Gene Description:
fibrillin 2
Transcript:
RefSeq NM_001999.3 (NON-CURRENT)
Match location:
Position 1995 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000433994)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAATGCAACATGGGTTATA
Hairpin Sequence:
5'-CCGG-GTGAATGCAACATGGGTTATA-CTCGAG-TATAACCCATGTTGCATTCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAATGCAACATGGGTTATACTCGAGTATAACCCATGTTGCATTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAATGCAACATGGGTTATACTCGAGTATAACCCATGTTGCATTCAC-3'

Other clones with same target sequence:

(none)