Construct: shRNA TRCN0000434004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023122.2-1329s21c1
DNA Barcode:
AGGATATGACCCTAGATTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpm6b (14758)
Gene Description:
glycoprotein m6b
Transcript:
RefSeq NM_023122.2 (NON-CURRENT)
Match location:
Position 1329 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGATATGACCCTAGATTTAC
Hairpin Sequence:
5'-CCGG-AGGATATGACCCTAGATTTAC-CTCGAG-GTAAATCTAGGGTCATATCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGATATGACCCTAGATTTACCTCGAGGTAAATCTAGGGTCATATCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGATATGACCCTAGATTTACCTCGAGGTAAATCTAGGGTCATATCCT-3'

Other clones with same target sequence:

(none)