Construct: shRNA TRCN0000434012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177263.3-960s21c1
DNA Barcode:
TACTACGGAAGGGACTGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zhx3 (320799)
Gene Description:
zinc fingers and homeoboxes 3
Transcript:
RefSeq NM_177263.3 (CURRENT)
Match location:
Position 960 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTACGGAAGGGACTGAAAT
Hairpin Sequence:
5'-CCGG-TACTACGGAAGGGACTGAAAT-CTCGAG-ATTTCAGTCCCTTCCGTAGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTACGGAAGGGACTGAAATCTCGAGATTTCAGTCCCTTCCGTAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTACGGAAGGGACTGAAATCTCGAGATTTCAGTCCCTTCCGTAGTA-3'

Other clones with same target sequence:

(none)