Construct: shRNA TRCN0000434020

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004925.3-825s21c1
DNA Barcode:
ATTGCGGGTGTCTTCGTGTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AQP3 (360)
Gene Description:
aquaporin 3 (Gill blood group)
Transcript:
RefSeq NM_004925.3 (NON-CURRENT)
Match location:
Position 825 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434020)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTGCGGGTGTCTTCGTGTAC
Hairpin Sequence:
5'-CCGG-ATTGCGGGTGTCTTCGTGTAC-CTCGAG-GTACACGAAGACACCCGCAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTGCGGGTGTCTTCGTGTACCTCGAGGTACACGAAGACACCCGCAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTGCGGGTGTCTTCGTGTACCTCGAGGTACACGAAGACACCCGCAAT-3'

Other clones with same target sequence:

(none)