Construct: shRNA TRCN0000434059

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021801.3-831s21c1
DNA Barcode:
TGTCCTTTCAGGGAGTTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MMP26 (56547)
Gene Description:
matrix metallopeptidase 26
Transcript:
RefSeq NM_021801.3 (NON-CURRENT)
Match location:
Position 831 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434059)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCCTTTCAGGGAGTTTATT
Hairpin Sequence:
5'-CCGG-TGTCCTTTCAGGGAGTTTATT-CTCGAG-AATAAACTCCCTGAAAGGACA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCCTTTCAGGGAGTTTATTCTCGAGAATAAACTCCCTGAAAGGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCCTTTCAGGGAGTTTATTCTCGAGAATAAACTCCCTGAAAGGACA-3'

Other clones with same target sequence:

(none)