Construct: shRNA TRCN0000434137

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003740.3-2256s21c1
DNA Barcode:
GTATTACTGAGCTCGGCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNK5 (8645)
Gene Description:
potassium two pore domain channel subfamily K member 5
Transcript:
RefSeq NM_003740.3 (NON-CURRENT)
Match location:
Position 2256 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434137)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATTACTGAGCTCGGCATTT
Hairpin Sequence:
5'-CCGG-GTATTACTGAGCTCGGCATTT-CTCGAG-AAATGCCGAGCTCAGTAATAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATTACTGAGCTCGGCATTTCTCGAGAAATGCCGAGCTCAGTAATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATTACTGAGCTCGGCATTTCTCGAGAAATGCCGAGCTCAGTAATAC-3'

Other clones with same target sequence:

(none)