Construct: shRNA TRCN0000434162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009781.3-3469s21c1
DNA Barcode:
AGAATGCAAGGGTAACTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacna1c (12288)
Gene Description:
calcium channel, voltage-dependent, L type, alpha 1C subunit
Transcript:
RefSeq NM_009781.3 (NON-CURRENT)
Match location:
Position 3469 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATGCAAGGGTAACTATAT
Hairpin Sequence:
5'-CCGG-AGAATGCAAGGGTAACTATAT-CTCGAG-ATATAGTTACCCTTGCATTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATGCAAGGGTAACTATATCTCGAGATATAGTTACCCTTGCATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATGCAAGGGTAACTATATCTCGAGATATAGTTACCCTTGCATTCT-3'

Other clones with same target sequence:

(none)