Construct: shRNA TRCN0000434215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207577.1-1752s21c1
DNA Barcode:
ATCTGTGTCACTGGTCGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP6 (4135)
Gene Description:
microtubule associated protein 6
Transcript:
RefSeq NM_207577.1 (CURRENT)
Match location:
Position 1752 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTGTGTCACTGGTCGATTT
Hairpin Sequence:
5'-CCGG-ATCTGTGTCACTGGTCGATTT-CTCGAG-AAATCGACCAGTGACACAGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTGTGTCACTGGTCGATTTCTCGAGAAATCGACCAGTGACACAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTGTGTCACTGGTCGATTTCTCGAGAAATCGACCAGTGACACAGAT-3'

Other clones with same target sequence:

(none)