Construct: shRNA TRCN0000434277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012243.1-1014s21c1
DNA Barcode:
GCAACCTCTTTATCGATAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC35A3 (23443)
Gene Description:
solute carrier family 35 member A3
Transcript:
RefSeq NM_012243.1 (NON-CURRENT)
Match location:
Position 1014 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACCTCTTTATCGATAATA
Hairpin Sequence:
5'-CCGG-GCAACCTCTTTATCGATAATA-CTCGAG-TATTATCGATAAAGAGGTTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACCTCTTTATCGATAATACTCGAGTATTATCGATAAAGAGGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACCTCTTTATCGATAATACTCGAGTATTATCGATAAAGAGGTTGC-3'

Other clones with same target sequence:

(none)