Construct: shRNA TRCN0000434301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172298.2-1611s21c1
DNA Barcode:
AGAGGAGGAGAAGTATGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tshz3 (243931)
Gene Description:
teashirt zinc finger family member 3
Transcript:
RefSeq NM_172298.2 (CURRENT)
Match location:
Position 1611 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGAGGAGAAGTATGATAT
Hairpin Sequence:
5'-CCGG-AGAGGAGGAGAAGTATGATAT-CTCGAG-ATATCATACTTCTCCTCCTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGAGGAGAAGTATGATATCTCGAGATATCATACTTCTCCTCCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGAGGAGAAGTATGATATCTCGAGATATCATACTTCTCCTCCTCT-3'

Other clones with same target sequence:

(none)