Construct: shRNA TRCN0000434310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002420.4-5156s21c1
DNA Barcode:
ATGATACTGTAACGAAGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM1 (4308)
Gene Description:
transient receptor potential cation channel subfamily M member 1
Transcript:
RefSeq NM_002420.4 (NON-CURRENT)
Match location:
Position 5156 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGATACTGTAACGAAGATAA
Hairpin Sequence:
5'-CCGG-ATGATACTGTAACGAAGATAA-CTCGAG-TTATCTTCGTTACAGTATCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGATACTGTAACGAAGATAACTCGAGTTATCTTCGTTACAGTATCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGATACTGTAACGAAGATAACTCGAGTTATCTTCGTTACAGTATCAT-3'

Other clones with same target sequence:

(none)