Construct: shRNA TRCN0000434362

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032606.3-1654s21c1
DNA Barcode:
TTGAGTCTGCATGGCTAATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAPS2 (84698)
Gene Description:
calcyphosine 2
Transcript:
RefSeq NM_032606.3 (NON-CURRENT)
Match location:
Position 1654 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434362)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGAGTCTGCATGGCTAATTC
Hairpin Sequence:
5'-CCGG-TTGAGTCTGCATGGCTAATTC-CTCGAG-GAATTAGCCATGCAGACTCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGAGTCTGCATGGCTAATTCCTCGAGGAATTAGCCATGCAGACTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGAGTCTGCATGGCTAATTCCTCGAGGAATTAGCCATGCAGACTCAA-3'

Other clones with same target sequence:

(none)