Construct: shRNA TRCN0000434440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009839.2-1534s21c1
DNA Barcode:
AGCAGGAATCTGGGATAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cct6b (12467)
Gene Description:
chaperonin containing Tcp1, subunit 6b (zeta)
Transcript:
RefSeq NM_009839.2 (NON-CURRENT)
Match location:
Position 1534 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGGAATCTGGGATAATTA
Hairpin Sequence:
5'-CCGG-AGCAGGAATCTGGGATAATTA-CTCGAG-TAATTATCCCAGATTCCTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGGAATCTGGGATAATTACTCGAGTAATTATCCCAGATTCCTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGGAATCTGGGATAATTACTCGAGTAATTATCCCAGATTCCTGCT-3'

Other clones with same target sequence:

(none)