Construct: shRNA TRCN0000434491

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017933.4-1221s21c1
DNA Barcode:
TGAGTAAGCAACGTTTCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PID1 (55022)
Gene Description:
phosphotyrosine interaction domain containing 1
Transcript:
RefSeq NM_017933.4 (NON-CURRENT)
Match location:
Position 1221 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434491)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGTAAGCAACGTTTCAAAT
Hairpin Sequence:
5'-CCGG-TGAGTAAGCAACGTTTCAAAT-CTCGAG-ATTTGAAACGTTGCTTACTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGTAAGCAACGTTTCAAATCTCGAGATTTGAAACGTTGCTTACTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGTAAGCAACGTTTCAAATCTCGAGATTTGAAACGTTGCTTACTCA-3'

Other clones with same target sequence:

(none)