Construct: shRNA TRCN0000434528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199459.3-761s21c1
DNA Barcode:
CCAAAGTATCAACACTAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C10orf71 (118461)
Gene Description:
chromosome 10 open reading frame 71
Transcript:
RefSeq NM_199459.3 (NON-CURRENT)
Match location:
Position 761 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGTATCAACACTAATTA
Hairpin Sequence:
5'-CCGG-CCAAAGTATCAACACTAATTA-CTCGAG-TAATTAGTGTTGATACTTTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGTATCAACACTAATTACTCGAGTAATTAGTGTTGATACTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGTATCAACACTAATTACTCGAGTAATTAGTGTTGATACTTTGG-3'

Other clones with same target sequence:

(none)