Construct: shRNA TRCN0000434656

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018440.3-1496s21c1
DNA Barcode:
TTAAGCTTCTGGACGAGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAG1 (55824)
Gene Description:
phosphoprotein membrane anchor with glycosphingolipid microdomains 1
Transcript:
RefSeq NM_018440.3 (NON-CURRENT)
Match location:
Position 1496 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434656)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAAGCTTCTGGACGAGAATG
Hairpin Sequence:
5'-CCGG-TTAAGCTTCTGGACGAGAATG-CTCGAG-CATTCTCGTCCAGAAGCTTAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAAGCTTCTGGACGAGAATGCTCGAGCATTCTCGTCCAGAAGCTTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAAGCTTCTGGACGAGAATGCTCGAGCATTCTCGTCCAGAAGCTTAA-3'

Other clones with same target sequence:

(none)