Construct: shRNA TRCN0000434677

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014212.3-874s21c1
DNA Barcode:
TCAACGTGTATATCAACAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HOXC11 (3227)
Gene Description:
homeobox C11
Transcript:
RefSeq NM_014212.3 (NON-CURRENT)
Match location:
Position 874 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434677)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAACGTGTATATCAACAAAG
Hairpin Sequence:
5'-CCGG-TCAACGTGTATATCAACAAAG-CTCGAG-CTTTGTTGATATACACGTTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAACGTGTATATCAACAAAGCTCGAGCTTTGTTGATATACACGTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAACGTGTATATCAACAAAGCTCGAGCTTTGTTGATATACACGTTGA-3'

Other clones with same target sequence:

(none)