Construct: shRNA TRCN0000434706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177790.4-1871s21c1
DNA Barcode:
GAACCTATCAGGCATATTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp385c (278304)
Gene Description:
zinc finger protein 385C
Transcript:
RefSeq NM_177790.4 (CURRENT)
Match location:
Position 1871 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTATCAGGCATATTTAC
Hairpin Sequence:
5'-CCGG-GAACCTATCAGGCATATTTAC-CTCGAG-GTAAATATGCCTGATAGGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTATCAGGCATATTTACCTCGAGGTAAATATGCCTGATAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTATCAGGCATATTTACCTCGAGGTAAATATGCCTGATAGGTTC-3'

Other clones with same target sequence:

(none)