Construct: shRNA TRCN0000434761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173812.4-2427s21c1
DNA Barcode:
ATAGTGTGTACAGAGTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPY19L2 (283417)
Gene Description:
dpy-19 like 2
Transcript:
RefSeq NM_173812.4 (NON-CURRENT)
Match location:
Position 2427 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAGTGTGTACAGAGTATTAA
Hairpin Sequence:
5'-CCGG-ATAGTGTGTACAGAGTATTAA-CTCGAG-TTAATACTCTGTACACACTAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAGTGTGTACAGAGTATTAACTCGAGTTAATACTCTGTACACACTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAGTGTGTACAGAGTATTAACTCGAGTTAATACTCTGTACACACTAT-3'

Other clones with same target sequence:

(none)