Construct: shRNA TRCN0000434817

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015980.3-915s21c1
DNA Barcode:
TTGTTCCCATGTAAGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NSG2 (51617)
Gene Description:
neuronal vesicle trafficking associated 2
Transcript:
RefSeq NM_015980.3 (NON-CURRENT)
Match location:
Position 915 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434817)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTTCCCATGTAAGATATTT
Hairpin Sequence:
5'-CCGG-TTGTTCCCATGTAAGATATTT-CTCGAG-AAATATCTTACATGGGAACAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTTCCCATGTAAGATATTTCTCGAGAAATATCTTACATGGGAACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTTCCCATGTAAGATATTTCTCGAGAAATATCTTACATGGGAACAA-3'

Other clones with same target sequence:

(none)