Construct: shRNA TRCN0000434851

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020631.3-3448s21c1
DNA Barcode:
TAGGCTGCCCATCCTACTTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEKHG5 (57449)
Gene Description:
pleckstrin homology and RhoGEF domain containing G5
Transcript:
RefSeq NM_020631.3 (NON-CURRENT)
Match location:
Position 3448 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434851)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGGCTGCCCATCCTACTTCT
Hairpin Sequence:
5'-CCGG-TAGGCTGCCCATCCTACTTCT-CTCGAG-AGAAGTAGGATGGGCAGCCTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGGCTGCCCATCCTACTTCTCTCGAGAGAAGTAGGATGGGCAGCCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGGCTGCCCATCCTACTTCTCTCGAGAGAAGTAGGATGGGCAGCCTA-3'

Other clones with same target sequence:

(none)