Construct: shRNA TRCN0000434875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021202.1-430s21c1
DNA Barcode:
TGGACGGCTGGCTCATCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP53INP2 (58476)
Gene Description:
tumor protein p53 inducible nuclear protein 2
Transcript:
RefSeq NM_021202.1 (NON-CURRENT)
Match location:
Position 430 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGACGGCTGGCTCATCATTG
Hairpin Sequence:
5'-CCGG-TGGACGGCTGGCTCATCATTG-CTCGAG-CAATGATGAGCCAGCCGTCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGACGGCTGGCTCATCATTGCTCGAGCAATGATGAGCCAGCCGTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGACGGCTGGCTCATCATTGCTCGAGCAATGATGAGCCAGCCGTCCA-3'

Other clones with same target sequence:

(none)