Construct: shRNA TRCN0000434918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014682.2-2497s21c1
DNA Barcode:
CTCTTATAGCTACGGTCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST18 (9705)
Gene Description:
ST18 C2H2C-type zinc finger transcription factor
Transcript:
RefSeq NM_014682.2 (CURRENT)
Match location:
Position 2497 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTTATAGCTACGGTCAATG
Hairpin Sequence:
5'-CCGG-CTCTTATAGCTACGGTCAATG-CTCGAG-CATTGACCGTAGCTATAAGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTTATAGCTACGGTCAATGCTCGAGCATTGACCGTAGCTATAAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTTATAGCTACGGTCAATGCTCGAGCATTGACCGTAGCTATAAGAG-3'

Other clones with same target sequence:

(none)