Construct: shRNA TRCN0000434933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006895.2-746s21c1
DNA Barcode:
CAATGCTAAGATGCTCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNMT (3176)
Gene Description:
histamine N-methyltransferase
Transcript:
RefSeq NM_006895.2 (NON-CURRENT)
Match location:
Position 746 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000434933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGCTAAGATGCTCATTAT
Hairpin Sequence:
5'-CCGG-CAATGCTAAGATGCTCATTAT-CTCGAG-ATAATGAGCATCTTAGCATTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGCTAAGATGCTCATTATCTCGAGATAATGAGCATCTTAGCATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGCTAAGATGCTCATTATCTCGAGATAATGAGCATCTTAGCATTG-3'

Other clones with same target sequence:

(none)