Construct: shRNA TRCN0000435007

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182506.2-731s21c1
DNA Barcode:
GAAGGAAGTGGAGCCTAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGEB10 (139422)
Gene Description:
MAGE family member B10
Transcript:
RefSeq NM_182506.2 (NON-CURRENT)
Match location:
Position 731 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435007)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGAAGTGGAGCCTAATAA
Hairpin Sequence:
5'-CCGG-GAAGGAAGTGGAGCCTAATAA-CTCGAG-TTATTAGGCTCCACTTCCTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGAAGTGGAGCCTAATAACTCGAGTTATTAGGCTCCACTTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGAAGTGGAGCCTAATAACTCGAGTTATTAGGCTCCACTTCCTTC-3'

Other clones with same target sequence:

(none)